BUSINESSGenotoxicity studies

The Item of Trusted Research Studies(Applicable to OECD, ICH, JMHW, MFDS)

Ames test using bacteria(Bacterial Reverse mutation Assay)

Ames test finds the returning mutate from tryptophan or histidine requirement to its opposite by using Frame shift mutagens《TA98, TA1537, TA1538》and Base pair substitution mutagens(TA 100, TA1535, WP2uvrA, WP2uvrA(Pkm101)) in other DNA that controls the histidine compound enzymes. This fast and simple method is the most conventional way of evaluating genotoxicity,which in turn generates reliable data by induction of Auto Clony counter

In vitro chromosome aberration test using cultivating cells of mammals(In vitro Chromosome Aberration Assay)

The test confirms if substances cause structural aberrations of chromosome in cultivated mammalian cells. The structural aberrations can be divided into chromosome abnormality and chromatic abnormality. Also, an increase in polyploidy may have the potential to induce numerical aberrations. In general. CHL (Chinese Hamster Lung)or CHO(Chinese Hamster Ovary) can be used to perform the test, and they are being used to find out the possibility of carcinogens and mutagens.

In vivo Micronucleus test using rodents(In vivo Micronucleus Assay)

In vivo Micronucleus test using rodents detects the damage of erythroblast' s chro-mosomes or the mitotic apparatus which can be caused by test substances through¬out the interpretation of red blood corpuscle gathered from bone marrow and/or peripheral blood cells.

Mouse Lymphoma TK Assay (Mouse Lymphoma TK Assay)

As an in vitro mammalian cell gene mutation testand. it serves to detect gene muta¬tions induced by chemicals. The major cell line is L5178Y mouse lymphoma cell, and by using thymidine kinase mutation as the endpoints, special characteristics of geno— toxicity is detected by observing the formation of trifluorothymidine(TFT) which can be expressed as L5178Y -3.7.2C TK+/-to TK-/-，resistant mutant, large colony and small colony